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Am J Vet Res. 2007 Sep;68(9):988-94.

Contribution of MX dynamin, oligoadenylate synthetase, and protein kinase R to anti-paramyxovirus activity of type 1 interferons in vitro.

Author information

1
Department of Pathology, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.

Abstract

OBJECTIVE:

To determine the contribution of MX dynamin, oligoadenylate synthetase (OAS), and double-stranded RNA-dependent protein kinase R (PKR) to the antiviral effects of type 1 interferons (IFNs) against bovine parainfluenza-3 virus (PI-3V) infection of Vero cells.

SAMPLE POPULATION:

Vero cell cultures.

PROCEDURES:

PI-3V yield was first compared between control and transfected type 1 IFNs-incompetent Vero cells expressing recombinant OAS or MX proteins. Afterwards, phosphorylation of eukaryotic initiation factor 2 alpha (eIF2alpha) was used to scale the degree of PKR activation upon infection of Vero cells by PI-3V.

RESULTS:

Overexpression of OAS did not result in significantly decreased viral replication. Phosphorylated eIF2alpha forms, the hallmark of PKR activation, were not increased in IFNalpha-primed infected Vero cells. Although human MXA contributed to partial blockade of replication of bovine PI-3V, the antiviral effect was not as strong as that of IFNalpha.

CONCLUSIONS AND CLINICAL RELEVANCE:

The powerful anti-Paramyxovirus activity of type 1 IFNs is mediated by noncanonic pathways.

PMID:
17764414
DOI:
10.2460/ajvr.68.9.988
[Indexed for MEDLINE]

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