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J Med Microbiol. 2007 Sep;56(Pt 9):1213-8.

Artemisinin triggers induction of cell-cycle arrest and apoptosis in Leishmania donovani promastigotes.

Author information

1
Department of Pharmacology, Institute of Postgraduate Medical Education & Research, 244 B Acharya J C Bose Road, Kolkata 700 020, India.

Abstract

A major impediment to effective anti-leishmanial chemotherapy is the emergence of drug resistance, especially to sodium antimony gluconate, the first-line treatment for leishmaniasis. Artemisinin, a sesquiterpene lactone isolated from Artemisia annua, is an established anti-malarial compound that showed anti-leishmanial activity in both promastigotes and amastigotes, with IC(50) values of 160 and 22 microM, respectively, and, importantly, was accompanied by a high safety index (>22-fold). The leishmanicidal activity of artemisinin was mediated via apoptosis as evidenced by externalization of phosphatidylserine, loss of mitochondrial membrane potential, in situ labelling of DNA fragments by terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labelling (TUNEL) and cell-cycle arrest at the sub-G(0)/G(1) phase. Taken together, these data indicate that artemisinin has promising anti-leishmanial activity that is mediated by programmed cell death and, accordingly, merits consideration and further investigation as a therapeutic option for the treatment of leishmaniasis.

PMID:
17761485
DOI:
10.1099/jmm.0.47364-0
[Indexed for MEDLINE]

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