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[The research for magnetic attachments producing static magnetic fields to influence on cytoskeletons of human periodontal ligament cells].

[Article in Chinese]

Author information

1
Department of Prosthodontics, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.

Abstract

OBJECTIVE:

The present research aimed to investigate that the static magnetic field (SMF) generated by dental magnetic attachments influenced on the cytoskeletons of human periodontal ligament cells (HPDLCs).

METHODS:

In vitro cultured HPDLCs were exposed to 10 mT and 120 mT SMF which simulated those of from the closed-field and open-field magnetic attachments respectively for 12-60 hours in a cellular SMF exposure system. The control group cells were cultured outside the SMF exposure system and were only exposed to geomagnetic field of about 0.03-0.07 mT. After finishing the SMF exposure, the cytoskeletons were observed under a laser scanning confocal microscope (LSCM). The image analysis software was utilized to measure and analyze the cell area, length/width ratios and the contents of F-actin of cells.

RESULTS:

There were certain changes observed in the cytoskeletons after HPDLCs exposed to 10 mT and 120 mT SMF for 12-60 hours. With the SMF strength increasing and the loading time extending, the cell microfilaments got ashorened change and a disordered arrangement inside cells, and the length/width ratios of cells decreased (P < 0.05). The areas of cells decreased after SMF loading for 60 hours (P < 0.05). The cell F-actin contents decreased after SMF loading for 12 hours (P < 0.05), but in the meantime there was no significant difference happening between the 36 or 60 hours SMF loading group and the control group (P > 0.05).

CONCLUSION:

In this study the SMF of magnetic attachments can lead the certain changes to the cytoskeletons in HPDLCs.

PMID:
17718441
[Indexed for MEDLINE]

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