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Nat Chem Biol. 2007 Oct;3(10):650-1. Epub 2007 Aug 23.

Light-controlled gene silencing in zebrafish embryos.

Author information

1
Department of Chemical and Systems Biology, Stanford University School of Medicine, 269 Campus Drive, CCSR 3155, Stanford, California 94305, USA.

Abstract

Functional genomic studies in zebrafish frequently use synthetic oligonucleotides called morpholinos that block RNA splicing or translation. However, the constitutive activity of these reagents limits their experimental utility. We report here the synthesis of a photoactivatable morpholino targeting the no tail (ntl) gene. This caged reagent permits spatiotemporal gene regulation in vivo and the photochemical generation of functionally mosaic organisms.

PMID:
17717538
DOI:
10.1038/nchembio.2007.30
[Indexed for MEDLINE]
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