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Mol Cancer. 2007 Aug 23;6:54.

mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer.

Author information

1
Department of Experimental and Diagnostic Medicine and Interdepartment Center for Cancer Research, University of Ferrara, Ferrara, Italy. lng@unife.it

Abstract

BACKGROUND:

Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome.

RESULTS:

We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H) for genome-wide expression of microRNA (miRNA) and mRNA. Based on combined miRNA and mRNA gene expression, a molecular signature consisting of twenty seven differentially expressed genes, inclusive of 8 miRNAs, could correctly distinguish MSI-H versus MSS colon cancer samples. Among the differentially expressed miRNAs, various members of the oncogenic miR-17-92 family were significantly up-regulated in MSS cancers. The majority of protein coding genes were also up-regulated in MSS cancers. Their functional classification revealed that they were most frequently associated with cell cycle, DNA replication, recombination, repair, gastrointestinal disease and immune response.

CONCLUSION:

This is the first report that indicates the existence of differences in miRNA expression between MSS versus MSI-H colorectal cancers. In addition, the work suggests that the combination of mRNA/miRNA expression signatures may represent a general approach for improving bio-molecular classification of human cancer.

PMID:
17716371
PMCID:
PMC2048978
DOI:
10.1186/1476-4598-6-54
[Indexed for MEDLINE]
Free PMC Article

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