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Curr Microbiol. 2007 Nov;55(5):389-95. Epub 2007 Aug 23.

PilJ localizes to cell poles and is required for type IV pilus extension in Pseudomonas aeruginosa.

Author information

1
Biology Department, University of Dayton, 300 College Park, Dayton, OH 45469-2320, USA.

Abstract

Twitching motility allows Pseudomonas aeruginosa to respond to stimuli by extending and retracting its type IV pili (TFP). PilJ is a protein necessary for this surface-associated twitching motility and bears high sequence identity with Escherichia coli methyl-accepting chemotaxis proteins (MCP). Here, we report that whereas wild-type P. aeruginosa PAO1 cells have extended pili at a single pole, pilJ mutant cells have shortened pili often at both poles despite normal levels of pilin accumulation, suggesting that PilJ is required for full TFP assembly/extension. Using yellow fluorescent protein fusions (pilJ-yfp), both plasmid born and in-frame chromosomal constructs, we determined that PilJ localizes to both poles of the cell. Overexpression of pilJ-yfp resulted in the protein accumulating between the poles.

PMID:
17713814
DOI:
10.1007/s00284-007-9008-5
[Indexed for MEDLINE]

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