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Proteomics. 2007 Sep;7(18):3259-63.

Antibody screening database for protein kinetic modeling.

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1
Molecular Translational Technology Section, Molecular Therapeutics Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

Abstract

Knowledge-based proteomic studies rely on the availability of quality antibodies. The increasing number of commercially available antibodies covers a wide range of protein networks; however, performance of each antibody can vary, depending on what type of cells, treatments, and time points are studied. Here, we describe an antibody database in which we screened 279 antibodies against multiple cell lysates after various treatments and from different time points. We applied these quality-confirmed antibodies on protein arrays, showing their utility for protein kinetic modeling.

PMID:
17708592
DOI:
10.1002/pmic.200700117
[Indexed for MEDLINE]
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