Format

Send to

Choose Destination
Appl Microbiol Biotechnol. 2007 Nov;77(1):13-22. Epub 2007 Aug 18.

Properties, production, and applications of camelid single-domain antibody fragments.

Author information

1
Institute for Animal Science and Health (ID-Lelystad) of Wageningen University and Research Centre, Edelhertweg 15, Lelystad, The Netherlands. michiel.harmsen@wur.nl

Abstract

Camelids produce functional antibodies devoid of light chains of which the single N-terminal domain is fully capable of antigen binding. These single-domain antibody fragments (VHHs or Nanobodies) have several advantages for biotechnological applications. They are well expressed in microorganisms and have a high stability and solubility. Furthermore, they are well suited for construction of larger molecules and selection systems such as phage, yeast, or ribosome display. This minireview offers an overview of (1) their properties as compared to conventional antibodies, (2) their production in microorganisms, with a focus on yeasts, and (3) their therapeutic applications.

PMID:
17704915
PMCID:
PMC2039825
DOI:
10.1007/s00253-007-1142-2
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center