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Cell Biochem Biophys. 2007;48(1):45-53.

Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains.

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1
Department of Biochemistry & Molecular Medicine, University of California Davis, Davis, CA 95616, USA.

Abstract

In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.

PMID:
17703067
[Indexed for MEDLINE]
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