Format

Send to

Choose Destination
Biotechnol Lett. 2008 Jan;30(1):145-51. Epub 2007 Aug 14.

Isolation and molecular characterization of a cryptic plasmid from Bifidobacterium longum.

Author information

1
Department of Food Science and Biotechnology, Kyungwon University, Seongnam 461-701, Korea.

Abstract

An isolate from the fecal samples of children was identified as Bifidobacterium longum. A plasmid isolated from it pBIFA24 was 4,892 bp with three open reading frames, ORFI, ORFII, and ORFIII. ORFI encoded a replication protein involved in a rolling-circle replication mechanism, and three sets of tandem repeat sequences featuring iteron structure were identified. Secondary structure prediction analysis of ORFII suggested it was a transmembrane protein. ORFIII showed high amino acid sequence identity with some mobilization proteins and contained an oriT sequence.

PMID:
17700996
DOI:
10.1007/s10529-007-9504-5
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center