Format

Send to

Choose Destination
See comment in PubMed Commons below
Nucleic Acids Res. 2007;35(16):5402-8. Epub 2007 Aug 13.

MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation.

Author information

1
Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.

Abstract

There is increasing evidence of crosstalk between epigenetic modifications such as histone and DNA methylation, recognized by HP1 and methyl CpG-binding proteins, respectively. We have previously shown that the level of methyl CpG-binding proteins increased dramatically during myogenesis leading to large-scale heterochromatin reorganization. In this work, we show that the level of HP1 isoforms did not change significantly throughout myogenic differentiation but their localization did. In particular, HP1gamma relocalization to heterochromatin correlated with MeCP2 presence. Using co-immunoprecipitation assays, we found that these heterochromatic factors interact in vivo via the chromo shadow domain of HP1 and the first 55 amino acids of MeCP2. We propose that this dynamic interaction of HP1 and MeCP2 increases their concentration at heterochromatin linking two major gene silencing pathways to stabilize transcriptional repression during differentiation.

PMID:
17698499
PMCID:
PMC2018631
DOI:
10.1093/nar/gkm599
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Silverchair Information Systems Icon for PubMed Central
    Loading ...
    Support Center