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Nat Methods. 2007 Sep;4(9):717-9. Epub 2007 Aug 12.

Tomographic phase microscopy.

Author information

1
G.R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue 6-014, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Abstract

We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.

PMID:
17694065
DOI:
10.1038/nmeth1078
[Indexed for MEDLINE]

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