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BMC Immunol. 2007 Aug 6;8:13.

Impact of polysialylated CD56 on natural killer cell cytotoxicity.

Author information

1
Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.

Abstract

BACKGROUND:

Siglec-7, a sialic acid binding inhibitory receptor expressed by NK cells is masked in vivo by a so far unknown ligand. It shows a strong binding prevalence for alpha-2,8-linked disialic acids in vitro.

RESULTS:

Here we describe the expression of PSA-NCAM (alpha-2,8-linked polysialic acid modified NCAM) on functional adult peripheral blood natural killer cells and examine its possible role in masking Siglec-7. Unmasking of Siglec-7 using Clostridium perfringens neuraminidase massively reduces NK cell cytotoxicity. By contrast a specific removal of PSA using Endo-NF does not lead to a reduction of NK cell cytotoxicity.

CONCLUSION:

The results presented here therefore indicate that PSA-NCAM is not involved in masking Siglec-7.

PMID:
17683591
PMCID:
PMC1976417
DOI:
10.1186/1471-2172-8-13
[Indexed for MEDLINE]
Free PMC Article

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