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Biochem Biophys Res Commun. 2007 Oct 5;361(4):974-9. Epub 2007 Jul 30.

A SWI/SNF- and INO80-dependent nucleosome movement at the INO1 promoter.

Author information

1
Department of Biology, College of Staten Island, City University of New York, Staten Island, NY 10314, USA.

Abstract

Transcriptional activation in yeast INO1 chromatin was studied using the indirect end-labeling technique. INO1 chromatin is organized into an ordered, overlapping nucleosomal array under repressing conditions. Nucleosome positions were only disrupted at the promoter region under inducing conditions in the presence of SWI/SNF and INO80. Mutants lacking either remodeler demonstrated identical positioning patterns as the wild type under repressing conditions. This indicates that these two remodelers are responsible and essential for local nucleosomal mobilization at the INO1 promoter. The area of local nucleosome movement is consistent with the previously identified region of histone deacetylation activity. In light of these findings, we suggest that nucleosomes subject to local mobilization are also targets for local histone modifications.

PMID:
17681272
PMCID:
PMC2034749
DOI:
10.1016/j.bbrc.2007.07.109
[Indexed for MEDLINE]
Free PMC Article

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