Effects of dietary manipulation on Alcian blue-positive (AB+) and periodic acid-Schiff-positive (PAS+) goblet cell populations were determined for the villi and intestinal crypts in the small intestine of early weaned pigs. Pigs were weaned at 21 days of age and samples from the distal portions of the duodenum and the middle and distal portions of the jejunum were obtained when pigs were 21, 24, 27, 30, 33, and 36 days old. Pigs were assigned to 1 of 3 dietary treatments; all diets contained 20% protein, on the basis of hydrolyzed casein, soybean meal, or corn-soybean meal. By day 24, three days after weaning, the populations of AB+ and PAS+ goblet cells were markedly decreased, regardless of diet. Goblet cell populations in the villi tended to increase from 3 to 15 days after weaning, whereas those in the intestinal crypts remained low throughout the study. Differences between any of the dietary treatments were not apparent for AB+ or PAS+ goblet cell populations in the villi or in the intestinal crypts. It appeared that early weaning per se, and not diet, was the primary cause for decreases in goblet cell populations.