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Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt 8):673-7. Epub 2007 Jul 21.

Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus.

Author information

1
Department of Chemical and Environmental Sciences and Materials and Surface Science Institute, University of Limerick, Limerick, Ireland.

Abstract

CzrB is a putative zinc transporter from Thermus thermophilus. The protein is proposed to consist of a hexahelical transmembrane domain with a cytosolic extramembranal C-terminus. The latter 92-residue fragment may be expressed free and may function independently of the full-length integral membrane protein. A 6xHis-tagged form of the water-soluble fragment has been overexpressed in Escherichia coli and diffraction-quality crystals of the tagged and tag-free variants have been grown. Preliminary X-ray analyses of tag-free fragment crystals with (2.2 A resolution) and without zinc ions (1.7 A resolution) reveal that the former has at least two zinc ions bound per monomer.

PMID:
17671365
PMCID:
PMC2335163
DOI:
10.1107/S1744309107032277
[Indexed for MEDLINE]
Free PMC Article

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