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J Am Acad Dermatol. 2007 Nov;57(5):824-31. Epub 2007 Jul 31.

Fluorescence diagnosis in keratinocytic intraepidermal neoplasias.

Author information

  • 1Department of Dermatology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands. t.smits@derma.umcn.nl

Abstract

BACKGROUND:

As different tissue types have distinct capabilities to accumulate protoporphyrin-IX, fluorescence diagnosis with aminolevulinic acid-induced porphyrin (FDAP) could be used to discriminate between different tissue types.

OBJECTIVE:

Protoporphyrin-IX accumulation and proliferation were studied in cutaneous squamous (pre)malignancies to see whether FDAP could be used to discriminate between different stages of keratinocytic intraepidermal neoplasia or proliferative status.

METHODS:

FDAP was performed in 14 patients (86 lesions) and biopsy specimens were taken, on which (immuno)histochemistry was performed for histopathologic classification and assessment of Ki67-antigen expression. Stratum corneum thickness was also measured.

RESULTS:

The fluorescence ratio (lesional:nonlesional skin) showed neither significant differences between the different keratinocytic intraepidermal neoplasia stages, nor between different levels of Ki67-antigen expression. Macroscopic fluorescence intensity and stratum corneum thickness were negatively correlated.

LIMITATIONS:

Relatively few malignancies were biopsied.

CONCLUSIONS:

With FDAP we were not able to discriminate between keratinocytic intraepidermal neoplasia lesions or proliferative activity. However, hyperkeratosis appeared to be an important determinant in variations in macroscopic fluorescence intensity.

PMID:
17669544
DOI:
10.1016/j.jaad.2007.06.031
[PubMed - indexed for MEDLINE]
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