Send to

Choose Destination
J Microbiol Methods. 2007 Oct;71(1):78-81. Epub 2007 Jul 10.

Construction and validation of a neutrally-marked strain of Pseudomonas fluorescens SBW25.

Author information

NZ Institute of Advanced Study and Institute of Molecular Biosciences, Massey University, Private Bag 102 904, North Shore Mail Centre, Auckland, New Zealand.


Neutrally marked bacterial strains are useful in many experimental evolution and molecular ecology studies to assess the relative fitness of a given strain. Here we describe the construction and validation of a neutral marker for the model organism Pseudomonas fluorescens SBW25. The marked strain, called SBW25-lacZ, was created by integrating a promoterless 'lacZ into the defective prophage locus of the SBW25 chromosome. Fitness assays conducted in various laboratory media and in planta revealed that the fitness levels of SBW25-lacZ were comparable with the wild-type ancestor.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center