Format

Send to

Choose Destination
J Microbiol Methods. 2007 Oct;71(1):78-81. Epub 2007 Jul 10.

Construction and validation of a neutrally-marked strain of Pseudomonas fluorescens SBW25.

Author information

1
NZ Institute of Advanced Study and Institute of Molecular Biosciences, Massey University, Private Bag 102 904, North Shore Mail Centre, Auckland, New Zealand. xx.zhang@auckland.ac.nz

Abstract

Neutrally marked bacterial strains are useful in many experimental evolution and molecular ecology studies to assess the relative fitness of a given strain. Here we describe the construction and validation of a neutral marker for the model organism Pseudomonas fluorescens SBW25. The marked strain, called SBW25-lacZ, was created by integrating a promoterless 'lacZ into the defective prophage locus of the SBW25 chromosome. Fitness assays conducted in various laboratory media and in planta revealed that the fitness levels of SBW25-lacZ were comparable with the wild-type ancestor.

PMID:
17669526
DOI:
10.1016/j.mimet.2007.07.001
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center