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Biochem Biophys Res Commun. 2007 Sep 14;361(1):127-32. Epub 2007 Jul 16.

The NMDAR subunit NR3A interacts with microtubule-associated protein 1S in the brain.

Author information

1
Karolinska Institutet, Department of Neurobiology, Care Sciences and Society, 141 86 Stockholm, Sweden. Maria.Eriksson@ki.se

Abstract

When screening a brain cDNA library, we found that the N-methyl-D-aspartate receptor subunit NR3A binds to microtubule-associated protein (MAP) 1S/chromosome 19 open reading frame 5 (C19ORF5). The interaction was confirmed in vitro and in vivo, and binding of MAP1S was localized to the membrane-proximal part of the NR3A C-terminus. MAP1S belongs to the same family as MAP1A and MAP1B, and was found to be abundant in both postnatal and adult rat brain. In hippocampal neurons the distribution-pattern of MAP1S resembled that of beta-tubulin III, but a fraction of the protein colocalized with synaptic markers synapsin and postsynaptic density protein 95 (PSD95), in beta-tubulin III-negative filopodia-like protrusions. There was coexistance between MAP1S and NR3A immunoreactivity in neurite shafts and occasionally in filopodia-like processes. MAP1S potentially links NR3A to the cytoskeleton, and may stabilize NR3A-containing receptors at the synapse and regulate their movement between synaptic and extrasynaptic sites.

PMID:
17658481
PMCID:
PMC3229768
DOI:
10.1016/j.bbrc.2007.06.179
[Indexed for MEDLINE]
Free PMC Article

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