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Biochem Biophys Res Commun. 2007 Sep 14;361(1):49-54. Epub 2007 Jul 20.

Plasmid encoded neurotoxin genes in Clostridium botulinum serotype A subtypes.

Author information

1
Department of Bacteriology, University of Wisconsin, 1925 Willow Drive, Madison, WI 53706, USA.

Abstract

Clostridium botulinum, an important pathogen of humans and animals, produces botulinum neurotoxin (BoNT), the most poisonous toxin known. We have determined by pulsed-field gel electrophoresis (PFGE) and Southern hybridizations that the genes encoding BoNTs in strains Loch Maree (subtype A3) and 657Ba (type B and subtype A4) are located on large (approximately 280 kb) plasmids. This is the first demonstration of plasmid-borne neurotoxin genes in Clostridium botulinum serotypes A and B. The finding of BoNT type A and B genes on extrachromosomal elements has important implications for the evolution of neurotoxigenicity in clostridia including the origin, expression, and lateral transfer of botulinum neurotoxin genes.

PMID:
17658467
PMCID:
PMC2346372
DOI:
10.1016/j.bbrc.2007.06.166
[Indexed for MEDLINE]
Free PMC Article

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