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Immunity. 2007 Jul;27(1):160-71. Epub 2007 Jul 19.

B cells acquire particulate antigen in a macrophage-rich area at the boundary between the follicle and the subcapsular sinus of the lymph node.

Author information

1
Lymphocyte Interaction Laboratory, Cancer Research United Kingdom London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom. ycarrasco@cnb.uam.es

Abstract

Adaptive B cell responses are initiated by B cell receptor-antigen engagement. Despite its importance, the early stages of B cell interaction with antigen in vivo remain elusive. Using fluorescent particulate antigen in combination with multiphoton microscopy and immune staining, we identified the follicle-subcapsular sinus (SCS) boundary of the lymph node as a site for B cell-antigen acquisition. In this macrophage-rich region, antigen accumulated shortly after administration, where specific follicular B cells were retained for long periods of time. These B cells acquired antigen cumulatively and became the main antigen carriers inside the follicle before polarizing to the B cell-T cell border. These observations not only provide evidence of a B cell role as antigen transporters into the follicle, but also highlight the boundary between the follicle and the SCS as a site for initiation of B cell responses.

PMID:
17658276
DOI:
10.1016/j.immuni.2007.06.007
[Indexed for MEDLINE]
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