Abstract
Microwave processors can provide a means of rapid processing and resin embedding for biological specimens that are to be sectioned and examined by transmission electron microscopy. This chapter describes a microwave-assisted protocol for processing, dehydrating, and embedding biological material, from living specimens to blocks embedded in sectionable resin in 4 h or less.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Epoxy Resins
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Fixatives
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Formaldehyde
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Glutaral
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Histological Techniques / methods
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Humans
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Mice
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Microscopy, Electron, Transmission / methods*
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Microwaves
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Osmium Tetroxide
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Plastic Embedding / methods*
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Saccharomyces cerevisiae / ultrastructure
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Tissue Fixation / methods
Substances
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Epoxy Resins
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Fixatives
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Formaldehyde
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Osmium Tetroxide
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Glutaral