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Molecular changes associated with teratogen-induced cyclopia.

Author information

1
Children's Memorial Research Center Program in Developmental Biology, Chicago, Illinois, USA.

Abstract

BACKGROUND:

Exposure of zebrafish embryos to a number of teratogens results in cyclopia, but little is known about the underlying molecular changes.

METHODS:

Using zebrafish embryos, we compare the effects cyclopamine, forskolin, and ethanol delivered starting just before gastrulation, on gene expression in early axial tissues and forebrain development.

RESULTS:

Although all three teratogens suppress gli1 expression, they do so with variable kinetics, suggesting that while suppression of Shh signaling is a common outcome of these three teratogens, it is not a common cause of the cyclopia. Instead, all teratogens studied produce a series of changes in the expression of gsc and six3b present in early axial development, as well as a later suppression of neural crest cell marker dlx3b. Ethanol and forskolin, but not cyclopamine, exposure reduced anterior markers, which most likely contributes to the cyclopic phenotype.

CONCLUSIONS:

These data suggest that each teratogen exposure leads to a unique set of molecular changes that underlie the single phenotype of cyclopia.

PMID:
17647295
DOI:
10.1002/bdra.20387
[Indexed for MEDLINE]

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