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Org Biomol Chem. 2007 Aug 7;5(15):2458-63. Epub 2007 Jun 27.

Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids.

Author information

1
Department of Chemistry, University of California-Davis, One Shields Avenue, Davis, CA 95616, USA.

Abstract

Sialyl Tn (STn) and sialyl lactoside derivatives containing O-acetylated sialic acid residues have been chemoenzymatically synthesized using a one-pot three-enzyme system and conjugated to biotinylated human serum albumin (HSA) using an adipic acid para-nitrophenyl ester coupling reagent. This approach provides an efficient and general protocol for preparing carbohydrate-protein conjugates containing base-sensitive groups.

PMID:
17637967
PMCID:
PMC2769491
DOI:
10.1039/b706507h
[Indexed for MEDLINE]
Free PMC Article

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