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PLoS One. 2007 Jul 18;2(7):e618.

Assembly of microtubules and actomyosin rings in the absence of nuclei and spindle pole bodies revealed by a novel genetic method.

Author information

1
Temasek Life Sciences Laboratory, National University of Singapore, Singapore; Department of Biological Sciences, National University of Singapore, Singapore.

Abstract

BACKGROUND:

The nucleus and the centrosomes (spindle pole bodies; SPBs in yeast) are believed to play key roles in the organization of various cellular structures, such as the actomyosin ring and microtubules. The ability to generate cells lacking nuclei and centrosomes (SPBs) is key to the elucidation of the role of these structures in various cellular processes.

METHODOLOGY/PRINCIPAL FINDINGS:

Here we describe a genetic method, using the Schizosaccharomyces pombe cdc16-116 mutant, to reliably and efficiently generate fission yeast cells lacking nuclei and SPBs. We use this approach to show that the assembly of microtubules does not require nuclear associated microtubule organizing centers and SPBs. We also show that actomyosin rings can assemble albeit inefficiently in the absence of nuclei and SPBs.

CONCLUSION:

We conclude that key cytoskeletal elements can be assembled in the absence of nuclei and SPBs. In addition, the approach we describe, taken together with physical approaches such as centrifugation, should facilitate the investigation of the role of the nucleus and SPBs in the assembly and inheritance of various cellular structures and organelles.

PMID:
17637834
PMCID:
PMC1913206
DOI:
10.1371/journal.pone.0000618
[Indexed for MEDLINE]
Free PMC Article

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