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Methods Mol Biol. 2007;375:95-106.

Methods for high-throughput materialization of genetic information based on wheat germ cell-free expression system.

Author information

1
Cell-Free Science and Technology Research Center, Ehime University, Matsuyama, Japan.

Abstract

Among the cell-free protein synthesis systems, the wheat germ-based translation system has significant advantages for the high-throughput production of eukaryotic multidomain proteins in folded state. Here, we describe protocols for this cell-free expression system.

PMID:
17634598
DOI:
10.1007/978-1-59745-388-2_5
[Indexed for MEDLINE]

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