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Nat Struct Mol Biol. 2007 Aug;14(8):696-703. Epub 2007 Jul 15.

The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex.

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1
Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N., Mailstop A1-162, Seattle, Washington 98109, USA.

Abstract

We incorporated the non-natural photoreactive amino acid p-benzoyl-L-phenylalanine (Bpa) into the RNA polymerase II (Pol II) surface surrounding the central cleft formed by the Rpb1 and Rpb2 subunits. Photo-cross-linking of preinitiation complexes (PICs) with these Pol II derivatives and hydroxyl-radical cleavage assays revealed that the TFIIF dimerization domain interacts with the Rpb2 lobe and protrusion domains adjacent to Rpb9, while TFIIE cross-links to the Rpb1 clamp domain on the opposite side of the Pol II central cleft. Mutations in the Rpb2 lobe and protrusion domains alter both Pol II-TFIIF binding and the transcription start site, a phenotype associated with mutations in TFIIF, Rpb9 and TFIIB. Together with previous biochemical and structural studies, these findings illuminate the structural organization of the PIC and the network of protein-protein interactions involved in transcription start site selection.

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PMID:
17632521
PMCID:
PMC2483787
DOI:
10.1038/nsmb1272
[Indexed for MEDLINE]
Free PMC Article

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