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Biophys J. 2007 Sep 15;93(6):L35-7. Epub 2007 Jul 13.

Dynamic patches of membrane proteins.

Author information

1
Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

Abstract

We test here a previously proposed hypothesis about lateral heterogeneity of cell membranes, a model predicting that heterogeneity is maintained by a combination of delivery and intake of molecules with barriers to lateral free diffusion. To test the validity of the model, we observed green fluorescent protein tagged major histocompatibility complex class I patches on the plasma membrane of mouse fibroblasts, using total internal reflection fluorescence microscopy in real time. The dynamic characterization revealed the life course of these patches comprises delivery of molecules at a short instant, followed by a slow, exponential decay, corresponding to diffusion of the molecules over dynamic barriers to free lateral diffusion. The characteristic lifetime of the patches, extracted from the measurements, is approximately 30 s, in excellent agreement with the predictions of the model.

PMID:
17631538
PMCID:
PMC1959560
DOI:
10.1529/biophysj.107.111567
[Indexed for MEDLINE]
Free PMC Article

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