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Appl Environ Microbiol. 2007 Sep;73(17):5692-7. Epub 2007 Jul 13.

Pathotyping Escherichia coli by using miniaturized DNA microarrays.

Author information

1
Department of Food and Environmental Safety, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. m.anjum@vla.defra.gsi.gov.uk

Abstract

The detection of virulence determinants harbored by pathogenic Escherichia coli is important for establishing the pathotype responsible for infection. A sensitive and specific miniaturized virulence microarray containing 60 oligonucleotide probes was developed. It detected six E. coli pathotypes and will be suitable in the future for high-throughput use.

PMID:
17630299
PMCID:
PMC2042074
DOI:
10.1128/AEM.00419-07
[Indexed for MEDLINE]
Free PMC Article
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