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J Proteome Res. 2007 Aug;6(8):3224-31. Epub 2007 Jul 13.

A novel approach to identify proteins modified by nitric oxide: the HIS-TAG switch method.

Author information

1
San Raffaele Scientific Institute, 20132 Milan, Italy. serena.camerini@iss.it

Abstract

S-nitrosylation is emerging as an important signaling mechanism that regulates a broad range of cellular functions. The recognition of Cysteine residues that undergo S-nitrosylation is crucial to elucidate how this modification modulates protein activity. We report here a novel strategy, defined His-tag switch, which allows the purification and identification of S-nitrosylated proteins and the unambiguous localization of the modified cysteine residues by mass spectrometry analysis.

PMID:
17629318
DOI:
10.1021/pr0701456
[Indexed for MEDLINE]

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