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Mol Cell Proteomics. 2007 Oct;6(10):1771-7. Epub 2007 Jul 11.

Assessing enzyme activities using stable isotope labeling and mass spectrometry.

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1
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Abstract

Activity-based protein profiling has emerged as a valuable technology for labeling, enriching, and assessing protein activities from complex mixtures. This is primarily accomplished via a two-step identification and quantification process. Here we show a highly quantitative and streamlined method, termed catch-and-release activity profiling of enzymes (CAPE), which reduces this procedure to a single step. Furthermore the CAPE approach has the ability to detect small quantitative changes that may have been missed by alternative mass spectrometry-based techniques.

PMID:
17627935
DOI:
10.1074/mcp.M700057-MCP200
[Indexed for MEDLINE]
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