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J Biotechnol. 2007 Aug 1;131(1):76-83. Epub 2007 Jun 6.

Collagen vitrigel membrane useful for paracrine assays in vitro and drug delivery systems in vivo.

Author information

1
Laboratory of Animal Cell Biology (currently, Transgenic Animal Research Center), National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. t.takezawa@affrc.go.jp

Abstract

We previously succeeded in converting a soft and turbid disk of type-I collagen gel into a strong and transparent vitrigel membrane utilizing a concept for the vitrification of heat-denatured proteins and have demonstrated its protein-permeability and advantage as a scaffold for reconstructing crosstalk models between two different cell types. In this study, we observed the nano-structure of the type-I collagen vitrigel membrane and verified its utility for paracrine assays in vitro and drug delivery systems in vivo. Scanning electron microscopic observation revealed that the vitrigel membrane was a dense network architecture of typical type-I collagen fibrils. In the crosstalk model between PC-12 pheochromocytoma cells and L929 fibroblasts, nerve growth factor (NGF) secreted from L929 cells passed through the collagen vitrigel membrane and induced the neurite outgrowth of PC-12 cells by its paracrine effect. Also, the collagen vitrigel membrane containing vascular endothelial growth factor (VEGF) showed sustained-release of VEGF in vitro and its subcutaneous transplantation into a rat resulted in remarkable angiogenesis. These data suggest that the collagen vitrigel membrane is useful for paracrine assays in vitro and drug delivery systems in vivo.

PMID:
17624459
DOI:
10.1016/j.jbiotec.2007.05.033
[Indexed for MEDLINE]

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