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Mol Ther. 2007 Oct;15(10):1820-6. Epub 2007 Jul 10.

A novel cell-penetrating peptide, M918, for efficient delivery of proteins and peptide nucleic acids.

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1
Department of Neurochemistry, Stockholm University, Stockholm, Sweden. samir@neurochem.su.se

Abstract

Cell-penetrating peptides (CPPs) have attracted increasing attention in the past decade as a result of their high potential to convey various, otherwise impermeable, bioactive agents across cellular plasma membranes. Albeit different CPPs have proven potent in delivery of different cargoes, there is generally a correlation between high efficacy and cytotoxicity for these peptides. Hence, it is of great importance to find new, non-toxic CPPs with more widespread delivery properties. We present a novel CPP, M918, that efficiently translocates various cells in a non-toxic fashion. In line with most other CPPs, the peptide is internalized mainly via endocytosis, and in particular macropinocytosis, but independent of glycosaminoglycans on the cell surface. In addition, in a splice correction assay using antisense peptide nucleic acid (PNA) conjugated via a disulphide bridge to M918 (M918-PNA), we observed a dose-dependent increase in correct splicing, exceeding the effect of other CPPs. Our data demonstrate that M918 is a novel CPP that can be used to translocate different cargoes inside various cells efficiently.

PMID:
17622242
DOI:
10.1038/sj.mt.6300255
[Indexed for MEDLINE]
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