Format

Send to

Choose Destination
Vet Res Commun. 2008 Jan;32(1):1-5. Epub 2007 Jul 5.

Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis.

Author information

1
Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università degli studi di Torino, Facoltà di Medicina Veterinaria, Via Leonardo da Vinci, 44, 10095 Grugliasco, TO, Italy.

Abstract

A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli. This technique, tested on isolates eae-positive, accurately detects eae and resolves alleles encoding the alpha1, alpha2, beta, gamma1, gamma2/theta, kappa, epsilon, zeta, and iota intimin variants.

PMID:
17611810
DOI:
10.1007/s11259-007-9009-7
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center