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J Clin Virol. 2007 Aug;39(4):266-70. Epub 2007 Jul 5.

Detection of differences in genomic profiles between herpes simplex virus type 1 isolates sequentially separated from the saliva of the same individual.

Author information

1
Department of Nutrition & Health Science, Faculty of Human Environmental Science, Fukuoka Woman's University, Fukuoka 813-8529, Japan. umene@fwu.ac.jp

Abstract

BACKGROUND:

Herpes simplex virus (HSV) is assumed to cause recrudescent lesions, usually through endogenous recurrence and rarely through exogenous re-infection. The occurrence of exogenous re-infection in genital and corneal HSV infections has been previously demonstrated using genomic analysis, while exogenous re-infection in oral-facial HSV infections has not been shown.

OBJECTIVES:

To confirm the occurrence of exogenous HSV re-infection in oral-facial HSV infections.

STUDY DESIGN:

Seven isolates (isolates 1-7) of herpes simplex virus type 1 (HSV-1) were sequentially separated from the same individual. Genomic profiles of HSV-1 isolates were studied: (i) by analysis of 20 RFLPs (restriction fragment length polymorphisms) and (ii) by the determination of nucleotide sequences of a PCR-amplified DNA fragment encompassing reiteration VII (hypervariable region) that belongs to sequences containing short tandem repeats.

RESULTS:

Isolates 1-5 were the same (F83 genotype) and isolates 6 and 7 were the same (F84 genotype), although isolates 1-5 were markedly different from isolates 6 and 7 in genomic profiles.

CONCLUSIONS:

The infection associated with isolates 6 and 7 was due to exogenous re-infection with F84 genotype virus, thus indicating the occurrence of exogenous HSV re-infection in oral-facial HSV infections.

PMID:
17611147
DOI:
10.1016/j.jcv.2007.05.012
[Indexed for MEDLINE]

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