Format

Send to

Choose Destination
Neuron. 2007 Jul 5;55(1):53-68.

Polarized signaling endosomes coordinate BDNF-induced chemotaxis of cerebellar precursors.

Author information

1
Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.

Abstract

During development, neural precursors migrate in response to positional cues such as growth factor gradients. However, the mechanisms that enable precursors to sense and respond to such gradients are poorly understood. Here we show that cerebellar granule cell precursors (GCPs) migrate along a gradient of brain-derived neurotrophic factor (BDNF), and we demonstrate that vesicle trafficking is critical for this chemotactic process. Activation of TrkB, the BDNF receptor, stimulates GCPs to secrete BDNF, thereby amplifying the ambient gradient. The BDNF gradient stimulates endocytosis of TrkB and associated signaling molecules, causing asymmetric accumulation of signaling endosomes at the subcellular location where BDNF concentration is maximal. Thus, regulated BDNF exocytosis and TrkB endocytosis enable precursors to polarize and migrate in a directed fashion along a shallow BDNF gradient.

PMID:
17610817
PMCID:
PMC2661852
DOI:
10.1016/j.neuron.2007.05.030
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center