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Methods Enzymol. 2007;423:458-75.

Single-cell analysis of gene expression by fluorescence microscopy.

Author information

1
Department of Physics, University of Pennsylvania, Philadelphia, PA, USA.

Abstract

Gene regulation by two-component systems has traditionally been studied using assays that involve averages over large numbers of cells. Single-cell measurements of transcription offer a complementary approach that provides the distribution of gene expression among the population. This chapter focuses on methods for using fluorescence microscopy and fluorescent proteins to study gene expression in single cells.

PMID:
17609146
DOI:
10.1016/S0076-6879(07)23022-8
[Indexed for MEDLINE]

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