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AIDS Res Hum Retroviruses. 2007 Jun;23(6):812-6.

Rapid MHC-II antigen presentation of HIV type 1 by human dendritic cells.

Author information

1
Division of Infectious Diseases, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4984, USA.

Abstract

HIV-1 is taken up by dendritic cells (DC) and degradation is detectable within a few hours. Little is known about how rapidly HIV-peptide-loaded MHC-II complexes appear on the surface of DC, however. A key impediment to the detailed understanding of MHC-II antigen presentation of HIV-1 has been the lack of good tools to quantitatively measure antigen presentation. We have developed HIV-1-gag p24 and reverse transcriptase (RT)-specific CD4(+) T cell hybridomas that demonstrate high sensitivity and specificity to detect HIV-1 antigens presented by MHC-II on human DC. We demonstrate that ex vivo primary blood myeloid DC (mDC) and monocyte-derived DC (MDDC) presented HIV-1 peptides on MHC-II molecules within 2 h of exposure to virions. HIV-1 was degraded in a compartment requiring acidification for processing and then was loaded onto newly synthesized MHC-II molecules for presentation.

PMID:
17604545
DOI:
10.1089/aid.2006.0280
[Indexed for MEDLINE]

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