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Pharm Res. 2008 Feb;25(2):475-80. Epub 2007 Jun 30.

Comparison of the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells.

Author information

1
Department of Pharmaceutics, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, New Jersey 08854, USA.

Abstract

PURPOSE:

To compare the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells.

MATERIALS AND METHODS:

PDZ proteins PDZK1 and NHERF1 were transfected into kidney LLC-PK1 cells and placental BeWo cells expressing hOAT4 or hOAT4-Delta, which lacks the PDZ consensus binding site. The interaction of PDZK1 and NHERF1 with hOAT4 and hOAT4-Delta was investigated by measurement of [3H] estrone sulfate uptake, cell surface and total cell expression of hOAT4.

RESULTS:

PDZK1 and NHERF1 enhanced hOAT4 activity in LLC-PK1 cells by increasing the cell surface expression of the transporter. In contrasts, these two PDZ proteins had no effect on hOAT4 activity in BeWo cells.

CONCLUSION:

The interaction of PDZ proteins with hOAT4 may be cell-specific. In placenta, a different set of interacting proteins from PDZK1 and NHERF1 may be required to modulate hOAT4 activity.

PMID:
17602283
DOI:
10.1007/s11095-007-9359-4
[Indexed for MEDLINE]

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