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Eur J Vasc Endovasc Surg. 2007 Sep;34(3):355-60. Epub 2007 Jul 2.

Tissue and urinary haemosiderin in chronic leg ulcers.

Author information

1
St. Thomas' Hospital, Academic Department of Surgery, Cardiovascular Division, 1st Floor, North Wing, Lambeth Palace Road, London SE1 7EH, UK. justin.tan@yahoo.co.uk

Abstract

OBJECTIVE:

The aim of this study was to assess the relationship between urinary and tissue haemosiderin in chronic leg ulcers, and its value as a diagnostic test for venous ulceration.

METHODS:

45 patients with chronic leg ulcers were recruited to the study (24 venous, 6 ischaemic, 6 lymphoedematous, 5 rheumatoid and 4 sickle cell). Punch biopsy of the ulcer edge was taken and early morning urine samples were collected. Positive Prussian-blue urinary haemosiderin granules were measured with a haemocytometer following Perls' staining. The percentage area of histological section staining positively with Perls' was measured using image analysis.

RESULTS:

84 urine samples and 46 ulcer biopsies were collected. Urinary haemosiderin was present in 92% of venous ulcer patients, but was absent in the ischaemic ulcer patients (p<0.0001). Significantly more urinary haemosiderin granules were detected in venous ulcer patients compared with patients who had lymphoedema (p<0.05). Tissue haemosiderin was detected in all ulcer types investigated. No correlation was found between the amounts of haemosiderin deposited in the tissue and the amount found in urine (r(2)=0.06).

CONCLUSIONS:

Haemosiderin is present in the urine of most patients with venous ulcers but not in ischaemia ulcers.

PMID:
17601755
DOI:
10.1016/j.ejvs.2007.02.006
[Indexed for MEDLINE]
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