Format

Send to

Choose Destination
See comment in PubMed Commons below
Anticancer Res. 2007 May-Jun;27(3B):1453-7.

Beclin 1 gene inhibits tumor growth in colon cancer cell lines.

Author information

  • 1Department of Gastroenterological Surgery, Faculty of Medicine, University of Fukui, Yoshida-gun, Fukui 910-1193, Japan.

Abstract

BACKGROUND:

The Beclin 1 gene binds to the apoptosis-inhibiting protein Bcl-2. The expression and function of the Beclin 1 gene in four colorectal cancer cell lines (HT29, DLD-1, SW480, SW620) was investigated.

MATERIALS AND METHODS:

The expression of Beclin 1 mRNA was examined by reverse transcription-polymerase chain reaction (RT-PCR), the cell growth rate of a stable cell line established after transfection of the Beclin 1 gene into low Beclin 1-expressing HT29 colon cancer cells. In addition, changes in cell cycle were investigated by flow cytometry, and the expression levels of cell cycle related proteins in the Beclin 1 transfected colon cancer cell line were assessed.

RESULTS:

The expression level of Beclin 1 mRNA in the colon cancer cell lines was variable. Transfection of the low Beclin 1 gene-expressing colon cancer cell line with the Beclin 1 gene resulted in cell growth inhibition. The cell cycle analysis showed that the percentage of GJ-phase cells was significantly higher in Beclin 1 transfectants than in mock transfected cells. The expression levels of cyclin E and phosphorylated Rb were decreased.

CONCLUSION:

These results indicate that Beclin 1 can inhibit the growth of colorectal cancer cells.

PMID:
17595761
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Support Center