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OMICS. 2007 Summer;11(2):166-85.

A network of genes regulated by light in cyanobacteria.

Author information

1
Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, St. Louis, Missouri 63104, USA. aurorar@slu.edu

Abstract

Oxygenic photosynthetic organisms require light for their growth and development. However, exposure to high light is detrimental to them. Using time series microarray data from a model cyanobacterium, Synechocystis 6803 transferred from low to high light, we generated a gene co-expression network. The network has twelve sub-networks connected hierarchically, each consisting of an interconnected hub-and-spoke architecture. Within each sub-network, edges formed between genes that recapitulate known pathways. Analysis of the expression profiles shows that the cells undergo a phase transition 6-hours post-shift to high light, characterized by core sub-network. The core sub-network is enriched in proteins that (putatively) bind Fe-S clusters and proteins that mediate iron and sulfate homeostasis. At the center of this core is a sulfate permease, suggesting sulfate is rate limiting for cells grown in high light. To validate this novel finding, we demonstrate the limited ability of cell growth in sulfate-depleted medium in high light. This study highlights how understanding the organization of the networks can provide insights into the coordination of physiologic responses.

PMID:
17594236
DOI:
10.1089/omi.2007.4323
[Indexed for MEDLINE]

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