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Nat Rev Mol Cell Biol. 2007 Aug;8(8):645-54.

Analysis of protein complexes using mass spectrometry.

Author information

1
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Ave, Room 992A, Toronto, Ontario, M5G 1X5, Canada. gingras@mshri.on.ca

Abstract

The versatile combination of affinity purification and mass spectrometry (AP-MS) has recently been applied to the detailed characterization of many protein complexes and large protein-interaction networks. The combination of AP-MS with other techniques, such as biochemical fractionation, intact mass measurement and chemical crosslinking, can help to decipher the supramolecular organization of protein complexes. AP-MS can also be combined with quantitative proteomics approaches to better understand the dynamics of protein-complex assembly.

PMID:
17593931
DOI:
10.1038/nrm2208
[Indexed for MEDLINE]

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