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Transplantation. 2007 Jun 27;83(12):1643-7.

Hyperexpression of Foxp3 and IDO during acute rejection of islet allografts.

Author information

1
Division of Nephrology and Hypertension, Department of Medicine, Weill Medical College of Cornell University, New York-Presbyterian Hospital, New York, NY 10021, USA.

Erratum in

  • Transplantation. 2007 Nov 27;84(10):1375. Thomas, Dolea A [corrected to Thomas, Dolca A].

Abstract

BACKGROUND:

We investigated the hypothesis that Foxp3+ cells are an integral component of antiallograft immunity but are dominated by pathogenic effectors.

METHODS:

Wild-type H-2b C57BL/6 (B6) mice or B6 mice with a targeted disruption of c-Rel gene (c-Rel-/-) were used as recipients of islet grafts from allogeneic DBA/2 (H-2d) mice or syngeneic B6 mice. We developed kinetic quantitative polymerase chain reaction assays and measured intragraft expression of mRNA for Foxp3, IDO, cytolytic molecules, proinflammatory cytokines, and chemokines/receptors.

RESULTS:

Intraislet levels of mRNA for Foxp3, IDO, CD3, CD25, tumor necrosis factor-alpha, RANTES, IP-10, and CXCR3 were highest in DBA/2 islet allografts from WT B6 recipients compared to DBA/2 islet allografts from c-Rel-/- B6 recipients or syngeneic B6 islet grafts from WT B6 mice. The ratio of granzyme B or IFN-gamma to Foxp3 was higher with the DBA/2 islet allografts from the WT B6 recipients compared to DBA/2 islet allografts from c-Rel-/- B6 recipients or B6 islet grafts from WT B6 recipients.

CONCLUSIONS:

Foxp3+ cells are an integral component of acute rejection of allografts but may be dominated by pathogenic effectors.

[Indexed for MEDLINE]

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