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J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jan 15;861(2):177-80. Epub 2007 Jun 7.

Purification and concentration of bacteriophage T4 using monolithic chromatographic supports.

Author information

1
BIA Separations d.o.o., Teslova 30, SI-1000 Ljubljana, Slovenia.

Abstract

Phages are gaining importance due to their wide usage. In this work strong anion exchange monolithic chromatographic column was used for single step phage purification. Most of the proteins and DNA were removed and recovery of approximately 70% of infective virus was reproducibly achieved. 30 ml of phage sample was purified in around 10 min.

PMID:
17588505
DOI:
10.1016/j.jchromb.2007.05.048
[Indexed for MEDLINE]

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