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Mol Cell Probes. 2007 Oct-Dec;21(5-6):400-4. Epub 2007 May 29.

Real-time PCR for the detection of Dientamoeba fragilis in fecal samples.

Author information

1
Leiden University Medical Center, Department of Parasitology, Postbus 9600, 2300 RC Leiden, The Netherlands. j.j.verweij@lumc.nl

Abstract

A real-time polymerase chain reaction (PCR) method targeting the 5.8S ribosomal RNA gene was developed for the detection of Dientamoeba fragilis in stool samples. The PCR also included an internal control to detect inhibition of the amplification by fecal constituents in the sample. The assay was performed on species-specific DNA controls (n=29) and a range of stool samples (n=85), and achieved high specificity and sensitivity. D. fragilis DNA could be detected in unpreserved fecal samples up to 8 weeks after storage at 4 degrees C. The use of this assay in a diagnostic laboratory offers the possibility of introducing DNA detection as a feasible technique for the routine diagnosis of intestinal D. fragilis infections.

PMID:
17587544
DOI:
10.1016/j.mcp.2007.05.006
[Indexed for MEDLINE]

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