Format

Send to

Choose Destination
Nano Lett. 2007 Jul;7(7):2043-5. Epub 2007 Jun 21.

Three-dimensional particle tracking via bifocal imaging.

Author information

1
Department of Biophysics, University of Illinois Urbana Champaign, Urbana, Illinois 61801, USA.

Abstract

We introduce a bifocal imaging method that enables three-dimensional (3D) tracking of both fluorescent and nonfluorescent particles. We accomplish this by simultaneously imaging a focused plane, for in-plane position (x,y), and a defocused plane, for out-of-plane position (z), of a molecule using a single CCD camera. We applied our method to several systems including in vivo melanosome tracking and phagocytosed fluorescent bead tracking. We have achieved 2-5 nm accuracy with a 2-50 ms time resolution.

PMID:
17583964
DOI:
10.1021/nl0709120
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center