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J Proteome Res. 2007 Aug;6(8):2978-92. Epub 2007 Jun 21.

Proteomic analysis of pancreatic zymogen granules: identification of new granule proteins.

Author information

1
Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA. rindlm01@med.nyu.edu

Abstract

The composition of zymogen granules from rat pancreas was determined by LC-MS/MS. Enriched intragranular content, peripheral membrane, and integral membrane protein fractions were analyzed after one-dimensional SDS-PAGE and tryptic digestion of gel slices. A total of 371 proteins was identified with high confidence, including 84 previously identified granule proteins. The 287 remaining proteins included 37 GTP-binding proteins and effectors, 8 tetraspan membrane proteins, and 22 channels and transporters. Seven proteins, pantophysin, cyclic nucleotide phosphodiesterase, carboxypeptidase D, ecto-nucleotide phosphodiesterase 3, aminopeptidase N, ral, and the potassium channel TWIK-2, were confirmed by immunofluorescence microscopy or by immunoblotting to be new zymogen granule membrane proteins.

PMID:
17583932
PMCID:
PMC2582026
DOI:
10.1021/pr0607029
[Indexed for MEDLINE]
Free PMC Article

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