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J Neurosci Methods. 2007 Aug 30;164(2):261-70. Epub 2007 May 16.

Preservation of glial cytoarchitecture from ex vivo human tumor and non-tumor cerebral cortical explants: A human model to study neurological diseases.

Author information

1
Department of Neurosurgery, Cancer Research Building, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Abstract

For the human brain, in vitro models that accurately represent what occurs in vivo are lacking. Organotypic models may be the closest parallel to human brain tissue outside of a live patient. However, this model has been limited primarily to rodent-derived tissue. We present an organotypic model to maintain intraoperatively collected human tumor and non-tumor explants ex vivo for a prolonged period of time ( approximately 11 days) without any significant changes to the tissue cytoarchitecture as evidenced through immunohistochemistry and electron microscopy analyses. The ability to establish and reliably predict the cytoarchitectural changes that occur with time in an organotypic model of tumor and non-tumor human brain tissue has several potential applications including the study of cell migration on actual tissue matrix, drug toxicity on neural tissue and pharmacological treatment for brain cancers, among others.

PMID:
17580092
PMCID:
PMC2744592
DOI:
10.1016/j.jneumeth.2007.05.008
[Indexed for MEDLINE]
Free PMC Article

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