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Zhong Yao Cai. 2007 Feb;30(2):185-8.

[Anti-oxidative effect of Angelica polysaccharide sulphate].

[Article in Chinese]

Author information

1
Department of Pharmacology, Fourth Military Medical University, Xi'an 710032, China.

Abstract

OBJECTIVE:

To investigate the anti-oxidative effect of Angelica polysaccharide sulphate( APS).

METHODS:

The Hela cells were cultured conventionally. Then APS was added and cultured together in different concentration for 24h followed by a oxidative injury with H2O2 or UV irradiation. The anti-oxidative effects of APS were detected as follow: cell viability was measured by MTT assay; colorimetric analysis was used to determine SOD activity, GSH and MDA level in cytoplasm.

RESULTS:

Treatment of H2O2 or UV irradiation significantly decreased cell viability, GSH and SOD activity in cytoplasm,while increased MDA in cytoplasm. At the range of 0. 3 -100microg/ml, APS significantly increased cell viabilty, GSH and SOD activity,while decreased MDA in a dose dependent manner( P < 0. 05 or P <0.01).

CONCLUSION:

APS has anti-oxidative effect,which may be one of its anti-AIDS mechanisms.

PMID:
17571770
[Indexed for MEDLINE]

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